Science & Data

Independent validation. West Texas conditions. Documented results.

The symBioGENESIS consortium platform is characterized by three independent laboratories across seven metagenomic analyses (2022–2025), then validated in the field by NELAP-accredited testing, university research, and multi-year trials across oilfield, wastewater, agriculture, and livestock applications.

This page is the evidence reference for B2B buyers evaluating a pilot or service contract. Every figure below is tied to a source document in our technical file.

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Independently validated by Eurofins NELAP Eurofins Midland Texas Tech University ServiTech Certified Labs Westbrook Field Trial
The Consortium

A naturally evolved platform — not a single-strain inoculant.

The symBioGENESIS consortium self-assembled over approximately one decade in outdoor digester environments in Mitchell County, Texas (E2 digester source). It is naturally fermented, not genetically engineered, and applied across verticals from a single fermentation without reformulation between applications.

Customer-facing materials reference 33 active species (organisms at ≥1% relative biomass; effective diversity equivalent e3.5 ≈ 33 from Shannon index 3.52). Scientific and technical contexts document 58 genomically characterized species (characterized across seven independent metagenomics reports, 2022–2025).

219,716
Annotated genes
Prokka annotation · CONSORTIUM_COMPREHENSIVE_BRIEF.pdf, Table 1
24
Functional categories
32,653 functionally categorized genes · Consortium Brief, §2.2
2,356+
Unique enzymes (EC-classified)
32-species core analysis · Consortium Brief, Table 1
min 2.3 × 105
CFU/mL (base concentrate)
Certified Labs, Report 22-416043 · BIOREVIVE_PQS_US_EN

Condition-dependent reorganization: Independent metagenomics (Texas Tech University, Lab+, Certified Labs) documented species-dominance shifts in response to oxygen gradients (October 2022 split-batch storage), NPK fertilization (March 2023), and petroleum exposure (August 2025) — without manual reformulation. The same production batch reorganized its community structure based on dissolved oxygen availability.

Colonization resistance: Diverse microbial communities suppress pathogens by consuming nutrients those pathogens require — a mechanism termed nutrient blocking (Spragge et al., Science, Vol. 382, 15 Dec 2023, DOI: 10.1126/science.aadj3502). This literature underpins safety and competitive-exclusion performance; Oxford did not test this specific product. Spragge et al., 2023 · cited in Consortium Brief §3.2 and WILDBIOME internal data brief
Field Validation

Evidence by vertical

Select your operation to review the supporting data. All figures include laboratory or trial attribution.

Oilfield & Remediation

West Texas sandy loam field conditions, Mitchell County, TX. BioRevive single application; semi-arid climate (~19.4 in/yr precipitation). Methods: TCEQ TX 1005 (TPH); SW846 8021B (BTEX+MTBE); SW846 6010D/7471A (metals).

29–83%
Total TPH (C6–C35) reduction at 35 days
Eurofins NELAP · Mitchell County, TX · BioRevive_TPH_Technical_Brief.md, Test 1
82–99%
Total TPH reduction at 98 days
Eurofins NELAP · Mitchell County, TX · Test 2 (Plot 2: 18,500 mg/kg starting TPH)
Non-detect
BTEX/VOC in 2 of 6 sub-plots within 25 days (Test 1); VOCs/BTEX non-detect throughout both studies
Eurofins · TPH Technical Brief, Tests 1–2
Non-detect
TPH, BTEX, and metals of concern in radish tissue after OBM-amended soil treatment
Eurofins crop tissue analysis · 29 May 2025 · TPH Technical Brief

Starting contamination context: up to 18,500 mg/kg TPH (Test 2, Plot 2). Results reflect West Texas sandy loam under semi-arid conditions with treatment-day irrigation only (no sustained supplemental irrigation). BioRevive is a biological process aid — not a pesticide. Preferential degradation of the C28–C35 paraffin fraction is an emerging, lab-stage application — the figures and full framing live on the commercial services page; it is not yet a field-validated wellbore service.

Validation Partners

Independent third-party validation

Results cited on this page come from external laboratories and universities — not internal marketing claims.

Eurofins NELAP (Midland, TX)

Environmental chemistry

TPH bioremediation (C6–C35), BTEX/VOC panels, metals, and crop-tissue safety on OBM-amended soils.

Methods: TCEQ TX 1005; SW846 8021B; SW846 6010D/7471A. Timeframe: 35- and 98-day Mitchell County field programs; Westbrook 30-day pesticide panel (atrazine, coumaphos, malathion).

Texas Tech University

Dr. Deborah Carr, 2019

Dissolved oxygen and BOD5 response on contaminated Colorado River water after sewage-spill scenario modeling.

Design: 8 treatment groups, 3 replicates, 5-day BOD5, ANOVA + TukeyHSD. Separate characterization: pH ~2.0 survivability.

ServiTech Laboratories

Soil fertility

Standard agronomic soil analysis documenting CEC improvement and soil-classification shift on BioRevive-treated plots.

Timeframe: Baseline Mar 2025 and one-year follow-up Mar 2026 (Mitchell County radish-growing area). Mehlich 3 ICP among standard panels.

Certified Labs

Pathogen & viability (formerly FSNS)

Monthly finished-product pathogen clearance and minimum APC viability for commercial concentrate.

Report 22-416043: min 2.3 × 105 CFU/mL. ISO 6579 / FDA-BAM pathogen panels: E. coli, Salmonella, Listeria negative since 2022 monitoring program.

Westbrook Field Trial

In-situ pesticide degradation

30-day field degradation of three target pesticides on Mitchell County, TX soils.

Compounds only: atrazine, coumaphos, malathion. Eurofins NELAP-accredited analysis per Consortium Brief Table 3.

USAMV Cluj-Napoca

Greenhouse floriculture

Controlled geranium trial comparing fertilizer-only vs. BIOTEEQ-adapted biology on shared Osmocote baseline.

Design: 200 plants, Feb–Apr 2026, 63 days. Preliminary descriptive means; full statistical replication trial underway.

Genomic Profile

Whole-genome architecture

Whole-genome sequencing and Prokka annotation (2022–2025) across seven metagenomic analyses and three laboratories. Naturally fermented — not genetically engineered.

219,716
Genes
24
Functional categories
2,356+
Unique enzymes
58
Characterized species
33
Active species (≥1% biomass)

Source: CONSORTIUM_COMPREHENSIVE_BRIEF.pdf, Tables 1–2 (March 2026)

Commercially relevant gene categories

  • Petroleum degradation: alkB and ladA genes across 25 species (Consortium Brief, Table 3)
  • Nitrogen cycling: 52 nitrogen-fixation genes across 10 species
  • Phosphorus metabolism: 11,732 genes (present in all 58 characterized species)
  • Pathogen suppression: colonization resistance via nutrient blocking (Spragge et al., Science 2023)
  • Enzyme diversity: lipase/esterase 1,510 genes; protease 2,897 genes

Production safety markers

  • Monthly pathogen panel: zero E. coli, Salmonella, Listeria, or Staph. aureus detections in finished product since 2022 (Certified Labs)
  • Feedstock vs. product: pathogens present in raw feedstock test below detection in finished concentrate (competitive exclusion in production)
  • Biosafety: BSL-1 / Risk Group 1 for all 58 sequenced species (CDC/NIH BMBL, 6th Ed.)
  • AMR screening: 261,917 proteins screened against CARD + ResFinder databases for clinically significant mobile resistance markers — none of NDM, KPC, MCR-1, or VanA detected. This is a specific-marker result, not a blanket “no resistance genes” claim (Consortium Brief §5)
Safety & Regulatory

Safety profile and regulatory positioning

  • OSHA HazCom 2012 (29 CFR 1910.1200): Not classified as hazardous. No GHS hazard pictograms; no signal word. (BIOREVIVE_SDS_US_EN)
  • FIFRA: Not a pesticide. Biological process aid; not registered and not required to register under FIFRA. No EPA pesticide registration claimed.
  • TSCA: Biological substances; components not subject to TSCA Inventory listing requirements.
  • California Proposition 65: Does not contain chemicals known to cause cancer, birth defects, or reproductive harm (SDS Section 15).
  • SDS availability: OSHA HazCom 2012-format Safety Data Sheet available on request (BIOT-SDS-US-2026-001). Direct public download link not yet published.
  • Pathogen safety: Monthly Certified Labs testing since 2022. Finished product: E. coli, fecal coliform, Salmonella, and Listeria not detected per standard panels.
  • Colonization resistance: Diverse communities suppress pathogens through nutrient competition (Spragge et al., Science, 2023, DOI 10.1126/science.aadj3502).
  • CFU (base concentrate): Minimum 2.3 × 105 CFU/mL (Certified Labs, Report 22-416043).
  • Microbiota+ / SOIL2: No AAFCO listing, FDA feed-additive approval, or TDA registration is claimed on this page. See pre-launch flags on agriculture and livestock tabs.
What we do not claim: EPA pesticide registration, FIFRA registration, AAFCO listing, or FDA feed-additive approval for any product line on this reference page.
References

Source documents & citations

  1. Spragge, F. J., et al. (2023). Microbiome diversity protects against pathogens by nutrient blocking. Science, 382(6676). DOI: 10.1126/science.aadj3502.
  2. Carr, D. (2019). An Analysis of Biological Oxygen Demand Response for Microbial Consortium Treatment to Sewage Spill in Colorado River. Texas Tech University. Structured data: 02_BIOREVIVE/ttu_validation/ttu_do_bod_results.json.
  3. BIOTEEQ Texas. BioRevive TPH Reduction & Sand-to-Soil Conversion Technical Summary Brief. Mitchell County, TX field program (Eurofins NELAP). 04_SERVICES/BioRevive_TPH_Technical_Brief.md.
  4. BIOTEEQ Texas. CONSORTIUM_COMPREHENSIVE_BRIEF.pdf (March 2026). Genomic architecture, Westbrook pesticide results, functional genome metrics. 06_WEBSITE/reference/consortium/.
  5. Eurofins Midland. Analytical job series 880-xxxxx (TPH, VOC, metals, crop tissue) cited in TPH Technical Brief.
  6. ServiTech Laboratories. Soil fertility panels (Lab 43827 baseline; 36011 one-year follow-up). Mitchell County, TX.
  7. Certified Labs (formerly FSNS). Report 22-416043 (APC viability); monthly pathogen monitoring program since 2022. 06_WEBSITE/reference/compliance/BIOREVIVE_PQS_US_EN (1).md.
  8. USAMV Cluj-Napoca & BIOTEEQ. Greenhouse geranium trial, Feb–Apr 2026. 06_WEBSITE/reference/product-info/BIOTEEQ_PITCH_CUSTOM_ADAPTED_GREENHOUSE_EN.html.
  9. Microbiota+ feedlot trial summary. 03_MICROBIOTA_PLUS/trials/Microbiota_brochure_draft.md (433 head, vet-managed, single-site).
  10. Cordos septic visual case study. 02_BIOREVIVE/cordos_case_study/ (visual/procedural time series; not a BOD/COD lab panel).
  11. BIOTEEQ Texas. BIOREVIVE_SDS_US_EN, BIOREVIVE_PQS_US_EN, BIOREVIVE_COC_US_EN. OSHA HazCom 2012 compliance set. 06_WEBSITE/reference/compliance/.
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